Skip to main content
. 2021 Nov 26;111(6):1147–1158. doi: 10.1002/JLB.3COVA0421-195RR

FIGURE 5.

FIGURE 5

ADAM17 inhibition affects mRNA levels of cytokines and adhesion molecules. Apratastat and TMI‐1 ameliorate the poly‐I:C/RBD‐S‐induced increases in TNF‐α mRNA levels (A), and TNF‐α serum protein levels (B). (C) ADAM 17 inhibitors induce an increase in IL‐1β mRNA. (D) IL‐6 mRNA levels are significantly reduced only after TMI‐1 treatment. (E) Apratastat and TMI‐1 further increase mRNA levels of IL‐10 compared with vehicle‐treated inflamed mice. The poly‐I:C/RBD‐S‐induced increase in mRNA levels of ICAM‐1 (F) and VCAM‐1 (G) are significantly reduced by treatment with both apratastat and TMI‐1. Gene expression was analyzed using quantitative real‐time RT‐PCR with β‐actin as housekeeping gene. Data are represented as mean ± SEM of at least 4 independent cDNA preparations per group. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001