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Sequences of the specific primers.
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| Collagen I | ACGTCCTGGTGAAGTTGGT | CAGGGAAGCCTCTCTCTCCT |
| FN | AATATCTCGGTGCCATTTGC | AAAGGCAT GAAGCACTCAA |
| SPARC | ATGACGACGGCACCTACAG | TCGCGTTGGGGTAACTTTTCA |
| CTGF | GCAGGCTAGAGAAGCAGAGC | ATGTCTTCATGCTGGTGCAG |
| GAPDH | ACAGTCAGCCGCATCTTC | CTCCGACCTTCACCTTCC |
FN, fibronectin; SPARC, secreted protein and rich in cysteine; CTGF, connective tissue growth factor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
