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. Author manuscript; available in PMC: 2022 Sep 28.
Published in final edited form as: Nat Cardiovasc Res. 2022 Mar 28;1(4):344–360. doi: 10.1038/s44161-022-00027-7

Fig. 1: Global Trpm2 deletion protects mice against atherosclerosis.

Fig. 1:

(a,b) Global Trpm2 deletion (Trpm2−/−) inhibited atherosclerotic plaque formation. a, Representative images of Oil Red O (ORO) staining of full-length aorta (red areas represents plaque). b, Mean atherosclerotic lesion ratio based on ORO staining from Trpm2+/+ (n=5) and Trpm2−/− mice (n=6), P < 0.0001. (c, d) Representative images and quantification of ORO staining of the aortic root sections from Trpm2+/+ (n=8) and Trpm2−/− mice (n=9), P = 0.0009. (e) Trpm2−/− did not influence the total cholesterol level in serum (n=5/group) (HFD: high-fat diet). (f) Trpm2−/− inhibited systemic inflammation evaluated by measuring IL-1β level in serum (n=4/group). (g-j) Trpm2−/− reduced macrophage burden in atherosclerotic plaque. g, h Representative merged images and quantification of Mac-1 staining of aortic root sections from Trpm2+/+ (n=7) and Trpm2−/− mice (n=8), P = 0.0027. (i, j) Representative merged images and quantification of F4/80 and CD80 staining of aorta cross-sections using the plaque areas as shown in Extended Data Fig. 1f (Red: F4/80; Blue: DAPI; Green: CD80) from Trpm2+/+ (n=9) and Trpm2−/− mice (n=8), P = 0.0001. (k, l) Trpm2−/− did not influence the leukocyte population in the peripheral blood. k, Representative monocyte population identified using flow cytometry. Ly6C+ monocyte population was identified from CD11b+ and Ly6G− leukocytes. l, Quantification of monocyte, neutrophil, B cell, T cell, NK cell and NKT cell population in the peripheral blood from Trpm2+/+ (n=5) and Trpm2−/− mice (n=5) with or without HFD treatment. (ns: no statistical significance; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ANOVA, two-tailed, Bonferroni’s test; mean ± SEM).