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. Author manuscript; available in PMC: 2022 Sep 28.
Published in final edited form as: Nat Cardiovasc Res. 2022 Mar 28;1(4):344–360. doi: 10.1038/s44161-022-00027-7

Extended Data Fig. 7. Inhibition of CD36 or TRPM2 did not produce additional inhibitory effect on M2KO macrophages.

Extended Data Fig. 7

(a) Representative WB analysis of the expression of CD36, pFyn, Fyn, pJNK, JNK, pp38 and p38 in isolated macrophages from M2KO mice after oxLDL treatment (50 μg/ml). Macrophages were treated with different inhibitors as indicated before protein extraction. (b) Quantification of WB bands (n=3/group). (c) Representative WB analysis of the expression of CD36, pFyn, Fyn, pJNK, JNK, pp38 and p38 in isolated macrophages from M2KO mice after TSP1 treatment (10 μg/ml). Macrophages were treated with different inhibitors as indicated before protein extraction. (d) Quantification of WB bands (n=3/group). (e) Representative WB analysis of the expression of iNOS in isolated macrophages from M2KO mice after oxLDL treatment (50 μg/ml). Macrophages were treated with different inhibitors as indicated before protein extraction. (f) Quantification of WB bands (n=3/group). (e) Representative WB analysis of the expression of iNOS in isolated macrophages from M2KO mice after TSP1 treatment (10 μg/ml). Macrophages were treated with different inhibitors as indicated before protein extraction. (f) Quantification of WB bands (n=3/group). (i) Representative WB analysis of the expression of MCP1 and MIF in isolated macrophages from M2KO mice after oxLDL treatment (50 μg/ml). Macrophages were treated with different inhibitors as indicated before protein extraction. (j) Quantification of WB bands (n=3/group). (k) Representative WB analysis of the expression of MCP1 and MIF in isolated macrophages from M2KO mice after TSP1 treatment (10 μg/ml). Macrophages were treated with different inhibitors as indicated before protein extraction. (l) Quantification of WB bands (n=3/group). (ns: no statistical significance; ANOVA, two-tailed, Bonferroni’s test; mean ± SEM).