. 2022 Apr 19;154:155889. doi: 10.1016/j.cyto.2022.155889

Table 1.

PCR and RFLP conditions for IL-6 polymorphisms identifications.

SNP locus	Primer sequences	Product size	Tm	Restriction enzyme	Fragment size
rs1800795 −174 G > C	F: TGCACTTTTCCCCCTAGTTGTGTCTTTC	202 bp	58 °C	Taq1	C allele: 202 bp Gallele:173 bp + 30 bp
rs1800795 −174 G > C	R: GAGCCTCAGACATCTCCAGTCCTAT	202 bp		Taq1	C allele: 202 bp Gallele:173 bp + 30 bp
rs1800796 − 634 G > C	F: GACTCAGTGGCAATGGGGAGAGC	606 bp	61 °C	BsrbI	Gallele:262 bp + 344 bp C allele: 606 bp
rs1800796 − 634 G > C	R: CGCTAAGAAGCAGAACCACTCTTCC	606 bp		BsrbI	Gallele:262 bp + 344 bp C allele: 606 bp
rs1800797 −597 A > G	F: GACTCAGTGGCAATGGGGAGAGC	606 bp	61 °C	BtscI	Aallele:236 bp + 370 bp G allele: 606 bp
rs1800797 −597 A > G	R: CGCTAAGAAGCAGAACCACTCTTCC	606 bp		BtscI	Aallele:236 bp + 370 bp G allele: 606 bp