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. 2022 Jan 25;59(4):2348–2362. doi: 10.1007/s12035-022-02756-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A 12-week fat-rich diet does not alter synaptic density in cortex and hippocampus. Cortical and hippocampal tissue was isolated from C57BL/6 mice, following 12-week feeding with obesogenic diets rich in palmitic acid (SFA; 45% total kCal) or oleic acid (MUFA; 45% total kCal), or a non-obesogenic, nutrient-matched control diet (chow; 10% total kCal from fat). Protein lysates were assessed for the expression of PSD-95, drebrin and synaptophysin (SYN) in cortex (a, c, e, g) and hippocampus (b, d, f, h), respectively. N = 3 animals per group. Data is presented as mean ± SEM overlaid with individual data points. Statistical differences were assessed using one-way ANOVA. Inserts illustrate representative immunoreactive bands for PSD-95, drebrin, SYN and β-actin (triplicate samples)