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. 2021 Jul 13;148(5):1137–1146. doi: 10.1007/s00432-021-03681-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Representative images of five types of CTCs isolated from NSCLC patients based on RNA-ISH of epithelial marker(red dots) and mesenchymal marker (green dots). A E-CTCs: epithelial marker + , mesenchymal marker-, CD45–, and DAPI + cells; B: hybrid E/M-CTCs: epithelial marker + , mesenchymal marker + , CD45–, and DAPI + cells; C M-CTCs: epithelial marker-, mesenchymal marker + , CD45–, and DAPI + cells; D E > m CTCs (the fluorescence dot counts of epithelial markers were double compared to the mesenchymal markers); E e = m CTCs (the fluorescence dot counts of epithelial markers were similar to mesenchymal markers); F e < M CTCs (the fluorescence dot counts of epithelial markers were two times lower than the mesenchymal markers)