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. 2022 Jan 24;148(5):1045–1055. doi: 10.1007/s00432-021-03854-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Impact of HS-5 stromal co-culture on proliferation and cluster size. A ATP content in RPMI-8226 cells culture in the 3D conical agarose microwell array as mono- vs. HS-5 co-culture. B Cluster volume of RPMI-8226 cells culture in the 3D conical agarose microwell array as mono- vs. HS-5 co-culture measured by transmitted-light scanner method. C Sketch showing cell aggregates in three microwells. This model uses the measuring cup principle for determination of cell proliferation. D Microscopy and scan of agarose matrix disk containing cell aggregates (100 × , 25 × and 1 × )