Fig. 5.

Modulation of Aβ deposition by E2F4DN in the cerebral cortex of 5xFAD mice. a Dense core plaques (Thioflavin S labeling, arrows) co-stained with Iba1 in the cerebral cortex of 3-month-old mice of the indicated genotypes. b Plaque size and plaque density in the cerebral cortex of 3-month-old mice of the indicated genotypes. c Percentages of the areas occupied by plaques in the indicated cortical layers at 3 months. d Thioflavin S labeling (arrows) co-stained with Iba1 in the cerebral cortex of 6 month-old mice of the indicated genotypes. e Plaque size and plaque density in the cerebral cortex of 6-month-old mice of the indicated genotypes. f Percentages of the areas occupied by plaques in the indicated cortical layers at 6 months. Numbers in a and d refer to the different cortical layers. Inserts show high magnifications of the indicated dashed boxes. *p < 0.05; **p < 0.01 (Student’s t test). 3 months: 5xFAD/EGFP (3 males and 1 female) and 5xFAD/E2F4DN (3 males and 1 female). 6 months: 5xFAD/EGFP (3 males and 1 females) and 5xFAD/E2F4DN (5 males). Scale bar: 100 μm