Fig. 2.

Cytotoxic effects of mibefradil in combination with chemotherapeutics. Concentration response curve and IC₅₀ values of vincristine alone or in combination with mibefradil in A D341 and C CHLA-01. Concentration response curve and IC₅₀ values of lomustine alone or in combination with mibefradil in E D341 and F CHLA-01 cells. Results for each concentration were standardised to the non-treated control and expressed as per cent control. B, D IC₅₀ statistical data comparison in (B) D341 and (D) CHLA-01. All the values are expressed as mean ± standard deviation of three independent experiment; n = 3, one-way ANOVA, with Dunnett multiple comparisons test compared with Vinc alone group, *p < 0.001 and **p < 0.0001. Mib, mibefradil. Vinc, vincristine. Lomu, lomustine