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. 2022 Apr 18;8:208. doi: 10.1038/s41420-022-01018-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A RT-qPCR results confirming overexpression of GBP2 at the mRNA level. B Western blotting results confirming overexpression of GBP2 at the protein level. C CCK8 analysis of cell proliferation in GFP- and GFP-GBP2-overexpressing U87 cells. D Colony formation analysis in GFP- and GFP-GBP2-overexpressing U87 cells. E Cell cycle analysis of U87 cells overexpressing GFP or GFP-GBP2 using PI staining. F Apoptosis analysis of GFP- and GFP-GBP2-overexpressing U87 cells using annexin V and PI. G Transwell invasion results of U87 cells overexpressing GFP or GFP-GBP2. Each experiment repeated as least 3 times. Magnification: ×10. ^*P < 0.05. ^**P < 0.01, ^***P < 0.001.