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. 2022 Apr 18;8:208. doi: 10.1038/s41420-022-01018-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Western blotting results confirming KIF22 knockdown efficiency, and showing expression of proteins related to EGFR signaling pathway in U87 and U251 cells transfected with siCtrl, siKIF22#1, or siKIF22#2. B CCK8 assay results for U87 and U251 cells transfected with siCtrl, siKIF22#1, or siKIF22#2. C, D Colony formation assay results for U87 and U251 cells transfected with siCtrl, siKIF22#1, and siKIF22#2. E Cell cycle assay results for U87 and U251 cells transfected with siCtrl, siKIF22#1, or siKIF22#2 using PI staining. F Apoptosis assay results for U87 and U251 cells using annexin V and PI staining. G Transwell invasion assay results for U87 and U251 cells. Each experiment repeated as least 3 times. Magnification: ×10. *P < 0.05, **P < 0.01, ***P < 0.001.