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. 2022 Apr 18;13(4):360. doi: 10.1038/s41419-022-04845-2

Fig. 3. MEOX2 overexpression promotes cell proliferation and motility in glioma.

Fig. 3

A MEOX2 protein expression in U87 and GSC91 cells transfected with MEOX2-Flag or empty vector lentivirus was detected by immunoblot. B CCK-8 assay was carried out to test the viability of MEOX2-overexpressed U87 cells or control cells. C Limiting dilution assay was performed in GSC91 cells with or without MEOX2 overexpression. D Representative images of sphere formation capability of GSC91 cells with or without MEOX2 overexpression. Bar = 100 µm. E EdU assay was utilized to measure cell proliferation of MEOX2-overexpressed U87 and GSC91 cells or control cells. Bar = 50 µm. F MEOX2-overexpressed U87 cells or control cells were intracranially injected into nude mice, and representative tumor xenografts of HE staining images are shown. G Effect of MEOX2 overexpression on U87 cell migration evaluated by wound healing assay. Bar = 400 µm. H The quantitative for the wound gap closure. I Invasion and migration capabilities were examined by transwell assay in U87 and GSC91 cells with or without MEOX2 overexpression. Bar = 200 µm. J The quantitative for the invasive and migrated cells number. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.