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. 2022 Apr 18;13(4):362. doi: 10.1038/s41419-022-04836-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Based on flat mount of retina, RGCs was marked by NeuN in green, multiple tiled images were captured and then assembled into a mosaic image of the whole retina flat mount using a confocal fluorescence microscope. Scale bars, 500 μm (upper panel), 200 μm (lower panel). B Using ImageJ, the cell density of RGCs labeled in green was calculated (WT-3M, 4065.5 ± 87.5, n = 6, E50K-3M, 3982.0 ± 66.1, n = 7, WT-16M, 3594.8 ± 137.9, n = 5, E50K-16M, 2027.9 ± 90.0, n = 7, *P < 0.05, **P < 0.01).