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. 2022 Apr 5;13:869050. doi: 10.3389/fimmu.2022.869050

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Copyright © 2022 Li, Wang, Xu, Che, Hu, Cao, Xie, Qiu, Shi, Liu, Dai and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Liraglutide pretreatment attenuates the apoptosis of liver cells caused by I/R. Mice were subjected to 60 min of hepatic ischemia, followed by 6 h of reperfusion. (A) TUNEL staining was performed, and TUNEL-positive cells were calculated (original magnification 400×). (B) The expression of Bax and Bcl-2 was determined via western blotting. Densitometric analysis of the Bax/β-actin (C), Bcl-2/β-actin (D) ratios is shown. All the results were obtained from at least three independent experiments. Data are presented as mean ± SD, n = 3 per group. ^**P < 0.01, ^***P < 0.001 vs. the Sham group; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs. the I/R group.