Figure EV4. Only reduced MAM content in neuroblastoma DX/REL pairs is correlated with attenuated mitochondrial responses to stress and chemotherapy resistance.

• A
  Mitochondrial biomass assessed by citrate synthase activity across DX/REL pairs; in triplicate from three biological replicates; mean with SD error bar shown.
• B
  Mitochondrial DNA content as quantified by qPCR using the mtDNA/nucDNA ratio for each of the two mitochondrial (MT‐C01 and MT‐ATPase6) and two nuclear (CFAP410 and MTTP) genes; in triplicate from three biological replicates; mean with SD error bar shown.
• C, D
  Electron micrograph images of heavy‐membrane mitochondria‐enriched fractions from CHLA15 and CHLA20 cells, identifying relative mitochondria abundance (identified within colored circles); quantification shown in D.
• E
  Heavy‐membrane fractions obtained from equal cell input were separated by Percoll gradient ultracentrifugation into MAM fractions (blue bars) and purified mitochondria for DX/REL pairs.
• F
  Immunoblot detection of MAM‐ and ER‐localized proteins in heavy‐membrane fractions from DX/REL pairs, with TOMM40 as a loading control for mitochondrial protein input.

Data information: Statistical analyses were performed using an unpaired two‐sided Student’s t‐test.