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. 2022 Mar 14;41(8):e109365. doi: 10.15252/embj.2021109365

Figure EV1. YAP/TAZ activation in secretory cells enforces fate conversion into AT1 cells, but bypasses basal or AT2 cells.

Figure EV1

  • A
    Representative IF images showing the expression of epithelial cell marker E‐cadherin and apoptosis marker TUNEL in Scgb1a1 lineage‐labeled tdTomato+ cells of control and Lats1/2 dKO lungs. Tomato (for secretory lineage, red), E‐cadherin (for epithelial cell, white), TUNEL (for apoptotic cells, green), and DAPI (blue). Scale bars, 100 μm.
  • B
    Representative IF images showing the expression of basal cell marker P63 in Scgb1a1 lineage‐labeled tdTomato+ cells in control and Lats1/2 dKO lungs. Tomato (for secretory lineage, red), P63 (green), and DAPI (blue). Scale bars, 100 μm.
  • C
    Representative IF images showing the expression of AT2 cell marker SPC in Scgb1a1 lineage‐labeled tdTomato+ cells in control and Lats1/2 dKO lungs. Tomato (for secretory lineage, red), SPC (green), and DAPI (blue). Scale bars, 100 μm.
  • D, E
    Representative IF images showing the expression of AT1 cell markers T1α (D), AGER (E), and endothelial marker VECAM (E) in Scgb1a1 lineage‐labeled tdTomato+ cells in control and Lats1/2 dKO lungs. Tomato (for secretory lineage, red), T1α (D, green), AGER (E, green), VECAM (E, white), and DAPI (blue). Scale bars, 100 μm.
  • F
    Quantification of Scgb1a1 lineage‐labeled tdTomato+AGER+ AT1 cells in (E). Data are presented as mean ± SEM (n = 5 mice for each group). *P < 0.05, ***P < 0.001 (Student’s t‐test).