Skip to main content
. 2022 Mar 14;41(8):e109365. doi: 10.15252/embj.2021109365

Figure EV2. YAP/TAZ signaling modulates the uptake of essential amino acids that are crucial for cell fate conversion of secretory cells.

Figure EV2

  • A
    Analysis of Gene Ontology (GO) terms in DATP cell states driven by YAP/TAZ activation.
  • B
    Heatmap of secretory, DATPs, and AT1 cells for metabolism‐related genes including hypoxia response, one‐carbon metabolism, and amino acid synthesis/serine/glycine metabolism.
  • C
    Experimental designs for organoid cultures derived from secretory cells isolated from unlabeled control and Lats1/2 dKO lungs. Isolated secretory cells were seeded in 3D organoid cocultures with stromal cells. At day 7 post seeding, 4‐hydroxytamoxifen (4‐OHT) was treated, followed by the addition of SLC7A5/LAT1 inhibitor (JPH203) twice every day for 7 days. Organoids were analyzed at day 7 post 4‐OHT treatment.
  • D
    Representative H&E and IF images of 3D organoids in (C). Tomato (red), CC10 (green), CAV1 (for AT1 cells, white), and DAPI (blue). Scale bars, 100 μm.
  • E, F
    Quantification of Scgb1a1 lineage‐labeled tdTomato+CC10+ secretory (E) and CAV1+ AT1 (F) cells in 3D organoids in (D). Data are presented as mean ± SEM (n = at least 2 technical replicates per group). *P < 0.05, **P < 0.01, NS, not significant (Student’s t‐test).