Figure EV5. Sustained YAP/TAZ‐mTORC1‐ATF4 signaling by chronic injury caused fate conversion of secretory cells into AT1 cells in the airways.

1. Experimental schematic diagram of naphthalene‐induced acute injury in Scgb1a1‐CreER^{TM /+} ;R26R^{tdTomato /+} mice post tamoxifen treatment.
2. Representative IF images showing the expression of YAP and TAZ, secretory cell marker CC10, DATP marker CLDN4, mTORC1 activity marker p‐S6, and ATF4 in Scgb1a1 lineage‐labeled tdTomato⁺ cells in control and naphthalene‐treated lungs. YAP/TAZ (green, 1^st panel), CC10 (green, 2^nd and 4^th panels), p‐S6 (white, 2^nd panel), CLDN4 (white, 3^rd panel), ATF4 (white, 4^th and 5^th panels). Scale bars, 100 μm.
3. Experimental schematic diagram of repetitive naphthalene‐induced chronic injury in Scgb1a1‐CreER^{TM /+} ;R26R^{tdTomato /+} mice post tamoxifen treatment.
4. Representative IF images showing the expression of AT1 cell marker AGER and DATP marker CLDN4 in Scgb1a1 lineage‐labeled tdTomato⁺ cells in control and naphthalene‐treated lungs. AGER (green), CLDN4 (white), and DAPI (blue). Scale bars, 100 μm.