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. 2020 Apr 21;225(8):1399–1410. doi: 10.1093/infdis/jiaa121

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© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Functional profiling of HB-101 vaccination-induced T cells. (A and B) Based on Boolean gating analysis, 15 populations representing all possible combinations of interferon (IFN)-γ, interleukin (IL)-2, tumor necrosis factor (TNF), and CD40L (CD4) or CD107a (CD8) were analyzed at the indicated time points. All vaccine recipients in the 3 cohorts were analyzed as 1 group, regardless of dose. Mean frequencies of CD8 and CD4 T cells specific for cytomegalovirus glycoprotein B (gB) (A) and pp65 (B) are displayed. *, Significant differences between placebo and vaccine groups at Month 4; **, significant differences between placebo and vaccine groups at Month 4 and 12. (C) Distribution of polyfunctional cytomegalovirus gB- and pp65-specific CD8 and CD4 T cells was analyzed using SPICE software. Mean proportions of cells producing 1, 2, 3, or 4 cytokines are shown at Day 28, Day 84, Month 4, and Month 12. Subpopulations with 4 functions are shown in black, with 3 functions in dark gray, bifunctional cells are shown in light gray and monofunctional cells are shown in white. Individual markers contributing to (multi)functionality are indicated by color-coded arcs.