Figure 3.

RNAseq from human pancreatic islets (n = 188). COBLL1 (A), SSTR5-AS1 (B), and ELN (C) were differentially expressed between diabetic donor islets compared to controls. The expression of COBLL1 (D) was negatively whereas those of SSTR5-AS1 (E) and ELN (F) were positively correlated with INS expression. ZDHHC14 (G) expression was upregulated whereas LCORL (H), EZH1 (I) and DBF4B (J) expression was downregulated in T2D compared to non-T2D donor islets, ZDDHC14 (K) expression correlated positively with insulin expression whereas EZHI (M) and DBF4B (N) correlated negatively. LCORL (L) showed no correlation. Normoglycemic islets (n = 31) were exposed to normal (5.5 mmol/L) and high (18.9 mmol/L) for 24 hours. ZDHHC14(O) expression was significantly upregulated whereas that of LCORL (P), EZH1 (Q) and DBF4B (R) was downregulated upon high glucose stimulation.