Figure 1. S1-RBD supports cell adhesion and proliferation.
A, FN-null MEFs (2.5×103 cells/cm2) were seeded onto coverslips pre-coated with S1-RBD (500 nM) and cultured for 4 h prior to fixation and phase-contrast imaging. Scale bar, 20 μm. B, FN-null MEFs (1.9×105 cells/cm2) were seeded onto tissue culture plates pre-coated with the indicated concentration of S1-RBD (filled circles) or FNIII10 (open circles). Cells were cultured for 90 min and relative cell number was determined by crystal violet staining. C, FN-null MEFs (1.9×105 cells/cm2) were seeded onto plates pre-coated with HN-tagged S1 (filled circles) or FNIII8–13 (open circles) for 90 min. Inset shows cell adhesion to S1 (filled circles) compared to BSA-coated wells (gray circle). D, FN-null MEFs (2.3×103 cells/cm2) were seeded onto tissue culture plates pre-coated with the indicated concentration of S1-RBD (filled circles), FNIII10 (open circles), or GST (gray circle) and cultured for 4 d. Relative cell number was determined by crystal violet staining. Data are mean ± SEM; n ≥ 3 experiments performed in triplicate.