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FN-null MEFs were either suspended in media or seeded at 3.5×105 cells/cm2 on wells pre-coated with 500 nM of S1-RBD or FNIII10, or 10 μg/ml of human plasma fibronectin (hFN) for 1 h. Cell lysates were analyzed by immunoblotting with the indicated phosphospecific antibodies. Molecular mass markers are shown at left
