Table 1. Summary of kinetic and quality control parameters determined for the interaction of αv integrins with immobilized S1-RBD and FNIII10.
Data are presented as mean ± SEM for at least 3 independent experiments per integrin. Double-referenced experiments were performed simultaneously for S1-RBD and FNIII10 ligands in parallel flow cells.
| Ligand | Analyte | ka × 104 | kd × 10−4 | KD (nM) | Rmax fit (RU) | Rmax measured (RU) | Chi2 |
|---|---|---|---|---|---|---|---|
| S1-RBD | αvβ3 | - | - | > 500 | - | - | - |
| S1-RBD | αvβ6 | 8.4 ± 1.8 | 236 ± 206 | 230 ± 180 | 6.9 ± 3.5 | 11.7 ± 0.7 | 0.1 ± 0.3 |
| FNIII10 | αvβ3 | 7.1 ± 1.4 | 15.0 ± 1.8 | 21.8 ± 1.6 | 12.8 ± 0.5 | 12.6 ± 0.4 | 0.06 ± 0.01 |
| FNIII10 | αvβ6 | 25.0 ± 18.8 | 6.0 ± 0.7 | 6.6 ± 3.1 | 16.6 ± 2.5 | 19.0 ± 2.3 | 0.16 ± 0.05 |