Production
envelopes of different growth-coupled design strategies
consisting of no more than five manipulations for lycopene. The production
envelope illustrates the minimum and maximum production rates a production
strain can achieve at different growth rates compared to the wild
type. The solid-blue production envelope is for the design strategy
using the minimal regulation set: ALCD19 (knockout), TKT2 (knockout),
DXPS (overexpressed), PItex (overexpressed), and TPI (underexpressed).
The dashed red production envelope is for the design strategy using
the maximal regulation set: FUM (knockout), R1PK (knockout), ADK3
(overexpressed), PItex (overexpressed), and ADK1 (underexpressed).
Reaction names are consistent with the genome-scale metabolic network
model of E. coli iML1515.