Figure 3.

Activity and cell-type specificity of library 1 heterotypic synthetic promoter constructs. Synthetic promoters (42) derived from in silico assembly of varying combinations of 2-mer homotypic TFRE blocks were placed upstream of a GFP reporter and transfected into a target DC line (DC 2.4); (A) or alternative off-target human cell lines (C2C12, NIH-3T3, CaCO-2, HUVEC); (B) synthetic promoter activity is shown relative to that observed using the human CMV-IE promoter control construct in each case. 11 synthetic promoters (starred) exhibiting >5% activity (marked by dotted line) in DC2.4 cells and minimal activities in the off-target cells are identified as the most DC-specific first library promoters. Values represent the mean ± standard deviation across three independent transfections, each performed in triplicate.