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. 2022 Apr 7;11(4):1613–1626. doi: 10.1021/acssynbio.2c00027

Figure 6.

Figure 6

In vitro screening of a second library of exclusively DC-specific promoters. Promoter constructs are screened for GFP expression relative to the hCMV-IE1 promoter (positive control). The observed RPU of all 16 second library promoters in the target DC2.4 cells and all four off-target cells (C2C12, NIH-3T3, CaCO-2, HUVEC). Promoters display activities ranging ∼20–61% in the target DC2.4 cells and <5% in all off-target cells. Values represent the mean ± standard deviation across three independent transfections, each performed in triplicate. (B) Schematic illustration of the architecture of the top three and bottom three second library promoters ranked RPU in the target DC2.4 cells, showing the differential representation of TFREs across both promoter categories.