Figure 3.

The genetic transformation system of squash was established using the strategy of optimal infiltration intensity. (a, b and c) GFP intensity, explants with infected vascular tissue, and survival rate of squash jingxinzhen No. 4 under five different treatments, respectively. Data are means of three replicates, and error bars indicate standard deviations. Different lowercase letters indicate significant differences (p < 0.05, Tukey’s test). (d) A regenerated transgenic T₀ plant of squash after one week of transfer to soil. The GFP-fluorescent tendril (e), apical meristem (f), and seeds (g) of a transgenic squash T₀ plant. A transgenic T₁ plant (h) and WT plant (i) of squash under the GFP channel of the LUYOR-3415RG.