Table 1.

The primers and PCR product size for 4 SNPs in the ADIPOQ gene

ID	Major/minor allele	Primer (sequence 5′ to 3′)	Restrection Enzyme	PCR Restrection	RFLP fragments (bp)
rs17300539	G/A	F: 5′-AGGCTCTGTGTGGACTGTGGA-3′ R: 5′-CCTGGAGAACTGGAAGCTGC-3′	MspI	296	AA = 296 GA = 129,167,296 GG = 129,167
rs266729	G/C	F: 5′-AGGCTCTGTGTGGACTGTGGA-3′ R: 5′-CCTGGAGAACTGGAAGCTGC-3’	HhaI	296	CC = 296 GC = 113,183,296 GG = 113,183
rs1501299	G/T	F: 5’- GGCCTCTTTCATCACAGACC-3′ R: 5′-AGATGCAGCAAAGCCAAAGT-3’	BsmI	196	TT = 196 GT = 50,146,196 GG = 50,146
rs17366743	T/C	F:5’-TAAGGGAGACATCGGTGAAAC-3′ R: 5′-TTACGCTCCTTCCCCATA −3’	Bst1107	438	CC = 438 TC = 104.334,438 TT = 104.334