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. 2022 Apr 19;41:148. doi: 10.1186/s13046-022-02354-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Dabrafenib- and dabrafenib+trametinib-induced SEMA6A-RhoA-YAP axis activation rescues actin cytoskeleton in BRAF-mut melanoma cells. A: BRAF-mut 2/59 and BRAF-wt ME4405 cells plated on poly-l lysine coated slides were treated or not with dabrafenib, trametinib and their combination for 48 h and 7 days, and stained with anti-YAP (green signal) and Phalloidin (red signal). The cells were counterstained with Hoechst to highlight nuclei. Scale bar 10 μm. B: Quantification of the subcellular localization of YAP from immunofluorescence of 2/59 and ME4405 cells untreated and treated with dabrafenib, trametinib and their combination for 48 h and 7 days. The results are reported as percentage of YAP expression in cytoplasmic + nuclear and nuclear fractions and at least 200 cells were counted per experiment. The results are presented as mean+/− standard deviation of three independent experiments (*p < 0.05; ** p < 0,001; *** p < 0,0001)