Figure 4.

Cortex and telencephalic hemispheres tagged by Neurog2^CreER(G2A)

(A) P7 cortical sections stained for nls-βGAL. The tagged neurons with green-stained nuclei are arranged inside-out across the cortical layers according to the TM injection stages shown at the top.

(B) P7 corpus callosum (between dotted lines) stained for mGFP. Note that axons tagged at TM12.5–TM13.5 and TM16.5–TM17.5 course through the ventral and dorsal parts of the callosum, respectively. See also Figure S1.

(C–F) mGFP staining of telencephalic sections from P7 mice tagged at TM11.5 (C), TM13.5 (D), TM15.5 (E), and TM17.5 (F). Different fiber systems and neurons are visualized depending on the stage of TM injection. (F′) A section stained for nls-βGAL from the same mouse as that in (F). The white line in (C) indicates the boundary between the neocortex (NCx) and the piriform cortex (PCx). In (F) and (F′), tagged neurons are more abundant in the somatosensory area (SS) than in the motor area (MO).

Scale bars, 200 μm (A and B) and 500 μm (C–F).