Table 1.
Detection antibodies used
Target | Labeling | Fluorescent | Isotype | Clone | Manufacturer | Catalog |
---|---|---|---|---|---|---|
CD9 | 5 μL | APC | mouse IgG1 | proprietary | Miltenyi Biotec | 130-108-813 |
CD63 | 5 μL | APC | mouse IgG1, κ | proprietary | Miltenyi Biotec | 130-108-813 |
CD81 | 5 μL | APC | REA | proprietary | Miltenyi Biotec | 130-108-813 |
CTLA-4 | 0.5 μg | APC | mouse IgG2a, κ | BNI3 | BioLegend | 369612 |
CD166 | 0.5 μg | APC | recombinant human IgG1 | REA442 | Miltenyi Biotec | 130-106-576 |
CD147 | 0.5 μg | APC | mouse IgG1, κ | HIM6 | BioLegend | 306214 |
CD87 | 0.5 μg | APC | recombinant human IgG1 | REA892 | Miltenyi Biotec | 130-114-851 |
CD90 | 0.5 μg | APC | mouse IgG1, κ | 5E10 | BioLegend | 328114 |
CD47 | 0.5 μg | APC | mouse IgG1, κ | CC2C6 | BioLegend | 323124 |
CD38 | 0.5 μg | APC | mouse IgG1, κ | HIT2 | BioLegend | 303510 |
CD15 | 0.5 μg | APC | mouse IgM, κ | HI98 | BioLegend | 301908 |
CD171 | 0.5 μg | APC | recombinant human IgG1 | REA163 | Miltenyi Biotec | 130-100-684 |
CD49F | 0.5 μg | APC | rat IgG2a, κ | GoH3 | BioLegend | 313616 |
VEGFR | 0.5 μg | APC | mouse IgG1κ | ES8-20E6 | Miltenyi Biotec | 130-093-601 |
EGFR | 0.5 μg | APC | mouse IgG1, κ | AY13 | BioLegend | 352906 |
EGFRvIII | 0.5 μg | AF647 | mouse IgG1, κ | DH8.3 | Novus Biologicals | NBP2-50599AF647 |
CD140 | 0.5 μg | APC | mouse IgG1, κ | 18A2 | BioLegend | 323608 |
CD10 | 0.5 μg | APC | mouse IgG1κ | 97C5 | Miltenyi Biotec | 130-093-450 |
CA9 | 0.5 μg | APC | recombinant human IgG1 | REA658 | Miltenyi Biotec | 130-110-058 |
CD314 | 0.5 μg | APC | mouse IgG1κ | 1D11 | BioLegend | 320808 |
IgG, immunoglobulin G; VEGFR, vascular endothelial growth factor receptor; EGFRvIII, epidermal growth factor receptor variant III.