REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD9	Miltenyi Biotec	Cat# 130-108-813
CD63	Miltenyi Biotec	Cat# 130-108-813
CD81	Miltenyi Biotec	Cat# 130-108-813
CTLA-4	BioLegend	Cat# 369612; RRID: AB_2632873
CD166	Miltenyi Biotec	Cat# 130-106-576; RRID: AB_2655529
CD147	BioLegend	Cat# 306214; RRID: AB_2750170
CD87	Miltenyi Biotec	Cat# 130-114-851; RRID: AB_2726804
CD90	BioLegend	Cat# 328114; RRID: AB_893431
CD47	BioLegend	Cat# 323124; RRID: AB_2716203
CD38	BioLegend	Cat# 303510; RRID: AB_314362
CD15	BioLegend	Cat# 301908; RRID: AB_314200
CD171	Miltenyi Biotec	Cat# 130-100-684; RRID: AB_2655584
CD49F	BioLegend	Cat# 313616; RRID: AB_1575047
VEGFR	Miltenyi Biotec	Cat# 130-093-601; AB_10828920
EGFR	BioLegend	Cat# 352906; AB_11150410
EGFRvIII	Novus Biologicals	Cat# NBP2-50599AF647; RRID: AB_2904021
CD140	BioLegend	Cat# 323608; RRID: AB_2162787
CD10	Miltenyi Biotec	Cat# 130-093-450; RRID: AB_10828545
CA9	Miltenyi Biotec	Cat# 130-110-058; RRID: AB_2651327
CD314	BioLegend	Cat# 320808; RRID: AB_492962
Biological samples
Human cerebral spinal fluid	National Institutes of Health (Dr. Steven Jacobson, Dr. Jennifer Jones)	N/A
Human plasma and serum	National Institutes of Health (Dr. Steven Jacobson, Dr. Jennifer Jones)	N/A
Chemicals, peptides, and recombinant proteins
Dulbecco Phosphate Buffered Saline	ThermoFisher Scientific	14190144
RPMI 1640 Medium	ThermoFisher Scientific	11875093
RPMI 1640 Medium (no phenol red)	ThermoFisher Scientific	11835030
McCoy's 5A (Modified) Medium	ThermoFisher Scientific	16600082
Critical commercial assays
MACSPlex Exosome Kit, human	Miltenyi Biotec	130-108-813
Deposited data
Example database	This publication	https://doi.org/10.5281/zenodo.5725951
Experimental models: Cell lines
PC3	Laboratory of Hisataka Kobayashi, NCI, NIH	N/A
PC3pip	Laboratory of Hisataka Kobayashi, NCI, NIH	N/A
U87	Laboratory of Kevin Camphausen, NCI, NIH	N/A
U251	Laboratory of Kevin Camphausen, NCI, NIH	N/A
HCT116 wt	Laboratory of Curt Harris, NCI, NIH	N/A
HCT116 mt	Laboratory of Curt Harris, NCI, NIH	N/A
HCT116 -/-	Laboratory of Curt Harris, NCI, NIH	N/A
UOK111	Laboratory of W. Marston Linehan, NCI, NIH	N/A
UOK151	Laboratory of W. Marston Linehan, NCI, NIH	N/A
Software and algorithms
MATLAB	Mathworks, Inc	https://www.mathworks.com/
MPAPASS Software	NIH	Original Code https://zenodo.org/record/5725534#.YZ7r-b3MIUE Standalone Software https://nano.ccr.cancer.gov/mpapass/
Other
qEVOriginal 70 nm	Izon Biosciences	N/A
qEV-10 70 nm	Izon Biosciences	N/A
JumboSep	PALL Corporation	FD100K65
Nanosight LM10	Malvern	N/A
CytoFLEX S	Beckman Coulter	N/A
Aurora	Cytek Biosciences	N/A
Optima XE ultracentrifuge	Beckman Coulter	B10049
45 titanium rotor	Beckman Coulter	339160