Figure 6.

ABT-263 Reduces fibrotic gene and protein expression in vivo. Expression of fibrogenic genes CTGF (A), TGFβ (B), and IGF-1 (C) in mice infected with S. typhimurium and treated daily with 20 or 100 mg/kg/day of ABT-263 compared with S. typhimurium-infected and uninfected controls (no drug or 100 mg/kg/day of ABT-263) as normalized to GAPDH gene expression. Representative westerns blots of collagen 1 (D) and αSMA (E). GAPDH expression was used a control for protein loading. E, Quantification of collagen 1 (F) and αSMA (G) normalized to GAPDH expression. Individual animals are represented by points within boxplots denoting the median and interquartile range. Statistical comparisons are enumerated within brackets with P values denoted.