REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
α-eGFP	ThermoFisher	#MA1-952; RRID:AB_889471
α-mouse	ThermoFisher	#32430; RRID:AB_1185566
α-GAPDH	ThermoFisher	#MA5-15738; RRID:AB_10977387
α-FLAG	Sigma	#F3165; RRID:AB_259529
Bacterial and virus strains
Escherichia coli OneShot Stbl3	ThermoFisher	Cat#C737303
Chemicals, peptides, and recombinant proteins
BocK	Fluorochem	Cat#078520
AzF	Bachem	Cat#4020250
OMeY	Alfa Aesar	H63096
Deposited data
Biological triplicates of flow cytometry results (∗.fcs) available in depository	This paper	https://doi.org/10.17035/d.2021.0135747960
Experimental models: Cell lines
HEK293	ECACC General Collection	Cat#85120602; RRID:CVCL_0045
Oligonucleotides
See Table S1 in the supplemental information	Merck Life Sciences	VC00021
Recombinant DNA
See vector cloning section below and the supplemental information for vector sequences STAR methods	This paper	n/a
Vector 58: Pyl tRNACUA(U25C) PylRS mCherry-P2A-eGFP(150TAG)	This paper	Addgene Plasmid #174526
Vector 62: Pyl tRNACUAG PylRS mCherry-P2A-eGFP(150CTAG)	This paper	Addgene Plasmid #174527
Vector 66: Pyl tRNAUCCU(Ev2) PylRS mCherry-P2A-eGFP(150AGGA)	This paper	Addgene Plasmid #174528
Vector 73: 4x(Pyl tRNAUCCU(Ev2)) PylRS mCherry-P2A-eGFP(150AGGA)	This paper	Addgene Plasmid #174529
Software and algorithms
FlowJo	BD Biosciences	RRID:SCR_008520
ZEN	Zeiss	RRID:SCR_013672
Image Lab	Bio-Rad	RRID:SCR_014210
Other
Dulbecco’s Modified Eagle Medium with GlutaMAX supplement	Fisher Scientific	#11574516
Fetal bovine serum	Fisher Scientific	#11573397
Trypsin-EDTA (0.25%)	Fisher Scientific	#11560626
Dulbecco’s Phosphate Buffered Saline	Sigma	#D1408
Lipofectamine 2000	Life Technologies	#10696343
Opti-MEM	Fisher Scientific	#11058021