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. 2021 Dec 6;1(8):100124. doi: 10.1016/j.crmeth.2021.100124

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Transformation efficiency and survival rate of electroporated protoplast of the UM1 strain

(A and B) The survival rate of the UM1 protoplast after electroporation after different ED (A) and TC pulses (B). Data is represented as mean ± SD. Each point corresponds to a different plate with 200 protoplasts (2 plates/cuvette).

(C and D) Transformation rate obtained after ED (C) and TC pulses (D). Data is represented as mean ± SD. Each point corresponds to a different electroporation cuvette.

(E) Transformation efficiency score was obtained as the product of the average transformants/μg of DNA and the average survival rate (normalized considering 1 as the value of the survival rate of the negative control).