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. 2021 Dec 6;1(8):100124. doi: 10.1016/j.crmeth.2021.100124

Figure 2.

Figure 2

Characterization of the transformants generated with the pMAT1819 plasmid

(A) Growth of the R. microsporus wild type strain ATCC 11559 (WT), UM1 strain, and a UM1 transformant-carrying plasmid pMAT1819 that complemented uracil auxotrophy (T1) on YNB medium supplemented with 5-FOA (3 g/L) and uridine (200 mg/L) (left), YNB medium supplemented with uridine (200 mg/L) (middle), and YNB medium (right).

(B) DNA was extracted from eight randomly selected transformants (T1–T8) and amplified by PCR using the M13_F and M13_R primers that bind to the vector sequence flanking the pyrF gene in plasmid pMAT1819. PCR amplification with the same primers of DNA from the untransformed UM1 strain and the purified plasmid served as negative and positive controls, respectively. Red arrow points to the expected amplification fragment. Black arrows point to the indicated bands of the marker.