REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
HLA-A2	Abcam	Clone: BB7.2; Cat# ab74674; RRID:AB_1280940
HLA-I	Abcam	Clone: W6/32; Cat# ab22432
HLA-I (in-house)	ATCC	HB-95 hybridoma
HLA-ABC-APC	BioLegend	Clone: W6/32; Cat# 311410; RRID:AB_314879
HLA-A02-PE	Invitrogen	Clone: BB7.2; Cat# 12-9876-42; RRID:AB_2784636
HLA-C-BV711	BD Biosciences	Clone: DT-9; Cat# 747594; RRID:AB_2744163
mTCRβ-PE	BioLegend	Clone: H57-597; Cat# 109208; RRID:AB_313431
hCD4-AF700	BD Biosciences	Clone: RPA-T4; Cat# 557922; RRID:AB_396943
hCD8-FITC	BioLegend	Clone: SK1; Cat# 344704; RRID:AB_1877178
hCD14-AF700	BD Biosciences	Clone: M5E2; Cat# 557923; RRID:AB_396944
hCD16-AF700	BD Biosciences	Clone: 3G8; Cat# 557920; RRID:AB_396941
hCD19-AF700	BD Biosciences	Clone: HIB19; Cat# 557921; RRID:AB_396942
hCD69-BV786	BD Biosciences	Clone: FN50; Cat# 563834; RRID:AB_2738441
CD14 MicroBeads, human	Miltenyi Biotec	Cat# 130-050-201; RRID:AB_2665482
CD25 MicroBeads, human	Miltenyi Biotec	Cat# 130-092-983
Biological samples
Human PBMCs from healthy donors	AllCells	N/A
Chemicals, peptides, and recombinant proteins
Heavy isotope-labeled synthetic peptides	Vivitide and BioNTech US Inc. (in-house)	N/A
FLT3L	Cellgenix	1015-050
TNF-α	Cellgenix	1006-050
IL-1β	Cellgenix	1011-050
PGE1	Cayman Pharma	CAS: 745-65-3
IL-7	Cellgenix	1010-050
IL-15	Cellgenix	1013-050
IncuCyte Annexin V red reagent	Sartorius	4641
Critical commercial assays
Chromium Single Cell 5’ Library & Gel Bead Kit	10x Genomics	PN-1000006
Chromium Single Cell V(D)J Enrichment Kit, Human T Cell	10x Genomics	PN-1000005
Chromium Single Cell A Chip Kit	10x Genomics	PN-120236
Chromium i7 Multiplex Kit	10x Genomics	PN-120262
PhiX Control	Illumina	FC-110-3001
MiSeq Reagent Kit v2 (300-cycles)	Illumina	MS-102-2002
U-PLEX Custom Biomarker (hu) Assays (IL-2, IFN-g, TNFa)	Meso Scale Diagnostics	K15067M-2
Deposited data
Mass spectrometry RAW files	https://massive.ucsd.edu/ProteoSAFe/static/massive.jsp	MSV000087987
TCR sequences	https://www.ncbi.nlm.nih.gov/nucleotide/	GenBank: MZ819175, MZ819176, MZ819177, MZ819178, MZ819179, MZ819180, MZ819181, MZ819182
R code for HLA-I sequence logo generation	Zenodo	https://doi.org/10.5281/zenodo.5222292
Experimental models: Cell lines
SW620	ATCC	Cat# CCL-227; RRID:CVCL_0547
A375	ATCC	Cat# CRL-1619; RRID:CVCL_0132
NCI-H441	ATCC	Cat# CRM-HTB-174; RRID:CVCL_1561
J.RT3-T3.5	ATCC	Cat# TIB-153; RRID:CVCL_1316
Lenti-X 293T	Takara Bio	Cat# 632180; RRID:CVCL_4401
Recombinant DNA
pCDH-CMV-MCS Lenti vector	System Biosciences	CD500B-1
pCDH-SFFV-Puro Lenti vector	In-house	N/A
pCDH-CMV-BSD Lenti vector	In-house	N/A
pCDH-CMV-Puro Lenti vector	In-house	N/A
MISSIOM lentiviral packaging plasmid mix	Sigma-Aldrich	SHP001
Software and algorithms
R	The R Foundation	Version 4.0.4
RStudio	RStudio	Version 1.4.1106
GraphPad Prism	GraphPad Software	Version 9.1.0; RRID:SCR_002798
Excel	Microsoft	Version 21.03; RRID:SCR_016137
HLAthena	Sarkizova et al. (2020)	http://hlathena.tools/
Interactive Peptide Spectral Annotator tool	Brademan et al. (2019)	http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html
Xcalibur	Thermo Scientific	Version 2.1.1565.23, 3.1.2412.25, or 3.3.2782.34; RRID:SCR_014593
Skyline-daily	University of Washington	Version 19.1.1.283 or newer; RRID:SCR_014080
Qual Browser Thermo Xcalibur	Thermo Scientific	Version 4.0.27.42
FreeStyle	Thermo Scientific	Version 1.6.75.20
FlowJo	BD Biosciences	Version 10.6.1; RRID:SCR_008520
Cell Ranger	10x Genomics	Version 2.0.2; RRID:SCR_017344
Loupe V(D)J Browser	10x Genomics	Version 2.0; RRID:SCR_018555
FACSDIVA	BD Biosciences	Version 8.0.1; RRID:SCR_018555
IncuCyte S3	Sartorius	Version 2018C; RRID:SCR_019874
MiSeq Control Software	Illumina	Version 2.6.2.1; RRID:SCR_016379
Meso Scale Discovery	Meso Scale Diagnostics	Version 4.0.12.1