TABLE 4.

Plasmids

Plasmid	Parent	Description	Relevant genotype	Reference or source
pPK15534		Suicide vector	GFP, Cmr, SpeI site	19
pPKΔZMO1754	pPK15534	ZMO1754 (aldB) knockout plasmid	GFP, Cmr, 1,000 bp insert up/down aldB	This work
pRL814		Broad host range plasmid	T7 lac GFP, Spr	26
pRLaldB	pRL814	Complementing plasmid	T7 lac aldB, Spr	This work
pRLHis-aldB	pRL814	Complementing plasmid	T7 lac His-aldB, Spr	This work
pET16b		E. coli expression vector	His-tag, Ampr	Novagen
pET16baldB	pET16b	aldB expression in E. coli	His-aldB, Ampr	This work