FIG 3.
NarQ169-NisK168 mediated nitrate-responsive expression in E. faecalis. Immunoblot analysis for MurAA of total protein lysates from exponentially growing E. faecalis cells. (A) E. faecalis grown with various concentrations of NaNO3 as indicated. (B) E. faecalis cells were untreated or grown with 25 ng/mL nisin or 0.5 mM NaNO3. The anti-MurAA antisera cross-reacts with a nonspecific lower band that serves as a loading control. Results are representative of a minimum of 2 independent biological replicates. Strains were wild-type, OG1/pJLL286; ΔmurAA, JL626/pJLL286; and ΔmurAA/PnisA-murAA, JL626/pJLL288.