FIG 5.
The rate of PG synthesis is reduced in the absence of MurAA. Incorporation of [14C] GlcNAc into SDS-insoluble peptidoglycan of exponentially growing E. faecalis was monitored following pulse-labeling for the ΔmurAA mutant (A) or the ΔmurAB mutant (B). The label incorporation rates calculated from data points between 10 and 25 min are shown in the tables below the graphs. Data represent mean ± standard deviation for two independent replicates. Strains were wild-type, OG1; ΔmurAA, JL626; ΔmurAB, CM1.