TABLE 3.
List of primers used
| Primer | Sequence (5′ to 3′)a |
|---|---|
| RpoE7:A:FP | CGGAATTCCATCAGTACGTAATGGCG |
| RpoE7:A:RP | GAAGATCTCTCCTGCTTGAGGCGCTG |
| RpoE7:B:FP | GAAGATCTCATGGTCACCAAGATGGC |
| RpoE7:B:RP | AACTGCAGGTGAAGAAATCCTTGGAC |
| Ltg-1:A:FP | AACTGCAGCAGAGATGCAGGCGCGAC |
| Ltg-1:A:RP | GAAGATCTCAGAAAGGAGGCGATCAGC |
| Ltg-1:B:FP | GAAGATCTCGCTTCGAAACGCTGACG |
| Ltg-1:B:RP | CGGAATTCCTTACCGGCATCAACGATC |
| RpoE7_pMMB:FP | CGGAATTCGATGGCGAACCACGTCGCG |
| RpoE7_pMMB:RP | AACTGCAGTCAATCGTCATCGGTCGG |
| Ltg-1_pMMB:FP | CGGAATTCCGTGGCCCTGGCCGCCGG |
| Ltg-1_pMMB:RP | AACTGCAGTCAGCGTTTCGAAGCGCCG |
| Ltg-1_032:FP | CCGCTCGAGGTGGCCCTGGCCGCCGGC |
| Ltg-1_032:RP | TCCCCCCGGGTCAGCGTTTCGAAGCGCCG |
| RpoE7_GSP1 | CGACGGATGCCGTTGATGTG |
| RpoE7_GSP2 | CTCCAGACATTCCTGCAC |
| RpoE7_GSP3 | ATCTCCGCCTCGATCTGCGCGATG |
| Oligo(dT) anchor primer | GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV |
| PCR anchor primer | GACCACGCGTATCGATGTCGAC |
| RpoE7_pCZ:FP | GCTCTAGAGCGGATGGATGAATGAGCG |
| RpoE7_pCZ:RP | CCCAAGCTTCGGTGGAACGCTGCCTCC |
| Ltg-1_pCZ:FP | GCTCTAGACGTAATGGCGCACCACAGG |
| Ltg-1_pCZ:RP | CCCAAGCTTGCGCTTTTCACCGTCTTGAC |
| AmpC_pCZ:FP | GCTCTAGAGAAGAGCCATTAGTTTTTCTTG |
| AmpC_pCZ:RP | CCCAAGCTTCGTGTCCTCGTCTCATGCC |
| RpoH1_032:FP | GGAATTCCATATGGCGACGATATCCAGCG |
| RpoH1_032:RP | AACTGCAGTCAACCGGCCAGCTTCTGC |
| RpoH2_032:FP | GGAATTCCATATGGCCTACATCGACGATCCCG |
| RpoH2_032:RP | AACTGCAGCTACGCGTCCGCCAGCAGATC |
| RpoH3_032:FP | GGAATTCCATATGACGGAACTGGCAGTTTCTG |
| RpoH3_032:RP | AACTGCAGGTCACACCGGCAGCAGCGC |
| RpoH4_032:FP | GGAATTCCATATGAGCACCGCATTGGCACTG |
| RpoH4_032:RP | AACTGCAGCCTCCCTCTTACGCCGCTGC |
| RpoH5_032:FP | GGAATTCCATATGCTCAAGAAACTCCTGACTGGTG |
| RpoH5_032:RP | CCGCTCGAGTCAGGCTGCCATGTGG |
| RpoH1_pMMB:FP | CGGAATTCATGGCGACGATATCCAGCG |
| RpoH1_pMMB:RP | AACTGCAGTCAACCGGCCAGCTTCTGC |
| RpoH2_pMMB:FP | CGGAATTCATGGCCTACATCGACGATCC |
| RpoH2_pMMB:RP | AACTGCAGCTACGCGTCCGCCAGCAG |
| RpoH3_pMMB:FP | CGGAATTCTTGACGGAACTGGCAGTTTC |
| RpoH3_pMMB:RP | AACTGCAGTCACACCGGCAGCAGCGC |
| RpoH4_pMMB:FP | CGGAATTCATGAGCACCGCATTGACGC |
| RpoH4_pMMB:RP | AACTGCAGTTATGCCGCTGCGGCGAG |
| RpoH5_pMMB:FP | CGGAATTCGTGCTCAAGAAACTCCTGAC |
| RpoH5_pMMB:RP | AACTGCAGTCAGGCTGCCATATGGGCC |
| RpoH3_pCZ:FP | GCTCTAGACCTCCATTGGCGGTCACTG |
| RpoH3_pCZ:RP | CCCAAGCTTCTCCTGATTCCGGCATCCTC |
| AmpC_002:FP | CCGCTCGAGGATGATCGGACGGCGGGCT |
| AmpC_002:RP | TCCCCCCGGGTCAGCCCTTGAGCGCGTC |
a
Underlined portions of sequence represent restriction enzyme sites used for cloning of insert.