FIG 2.

Supplementation of the thiamin mutants of E. coli K-12 MG1655 with thiamin, HMP, and THZ in M9 medium. (A to D) Growth phenotype of the wild-type strain (A), the EcΔthiC strain (B), the EcΔthiE strain (C), and the EcΔthiG strain (D). The following symbols in panels A to D represent the indicated concentrations of thiamin: □, nothing added; ○, 1 pM; Δ, 1 nM; ∇, 100 nM; ◊, 1 μM; *, 20 μM. (E to G) Supplementation of the EcΔthiC mutant (E), the EcΔthiG mutant (F), and the EcΔthiE mutant (G). Symbols in panels E to G: ∇, no HMP/THZ; □, 100 nM HMP; Δ, 100 nM THZ; ○, 100 nM thiamin. Means ± standard errors of the means of results from three independent experiments are plotted.