FIG 5.

Mixed-species cocultures of the thiamin biosynthesis mutants of V. anguillarum PF430-3 and E. coli K-12 MG1655 in thiamin-deficient M9 medium. Mixed cocultures of the VaΔthiC-EcΔthiE strains (A), the VaΔthiE-EcΔthiC strains (B), the VaΔthiC-EcΔthiG strains (C), the VaΔthiG-EcΔthiC strains (D), the VaΔthiE-EcΔthiG strains (E), and the VaΔthiG-EcΔthiE strains (F) without thiamin supplementation. All empty gray and black symbols represent single cultures of V. anguillarum and E. coli, respectively. All filled symbols represent the cocultures, with gray and black colors representing the corresponding reverse combinations. Means ± standard deviations of results from three independent experiments are plotted.