FIG 2.
Control of membrane fluidity is not the primary role of TrhAEC. (A) Total fatty acid composition of E. coli WT and the ΔtrhAEC strain grown at 37°C. (B) Total fatty acid composition of E. coli WT and the ΔtrhAEC strain grown at 16°C. Error bars represent the standard deviation of two biological and two technical replicates. P values are listed above each set of bars. (C) Phospholipid headgroup analysis of E. coli WT and the ΔtrhAEC strain. The percent compositions of phosphatidylethanolamine (PE) lipids, diphosphatidylglycerol (PG) lipids, and diphosphatidylglycerol (DPG)/cardiolipin lipids are the averages from three biological replicates. (D) Membrane fluidity of E. coli WT and the ΔtrhAEC mutant. The membrane fluidity of whole cells was measured using DPH anisotropy, where greater values in anisotropy indicate a more rigid membrane. The data represent six biological replicates, where each point is the average of three technical replicates. Error bars represent the standard deviation.