FIG 3.
Membrane proteome of the ΔtrhAEC mutant reveals large changes in protein abundance, cellular physiology, and metabolism. (A) Venn diagram representing numbers of the differentially abundant proteins in the membrane proteome of ΔtrhAEC relative to E. coli WT. (B) Heat map of proteins that are found in decreased or increased abundance at both 37°C and 16°C in the membrane proteome of ΔtrhAEC relative to E. coli WT. The average log2 fold change of six biological replicates is represented. (C and D) Representation of proteins that are found in decreased abundance in the membrane proteome of ΔtrhAEC relative to WT E. coli at (C) 37°C and (D) 16°C. Proteins are functionally classified into GO-terms. (E and F) Representation of proteins that are found in increased abundance in the membrane proteome of ΔtrhAEC relative to WT E. coli at (E) 37°C and (F) 16°C.