FIG 5.
Cells lacking TrhAEC have a depolarized membrane. (A and B) The membrane potential of E. coli WT and the ΔtrhAEC strain was measured using (A) ThT and (B) DiOC2(3) fluorescent reporters. DMSO treatments represent the resting membrane potential of cells. The membrane potential of each strain was collapsed when treated with CCCP, as indicated by decreased reporter fluorescence. The data represent fluorescence from three biological replicates with three technical replicates. Error bars represent the standard deviation.