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Secretion and expression of ESX-1-associated proteins in liquid culture media over time. Immunoblots of CF (10 μg/well) and CL proteins (5 μg/well) of wild-type M. tuberculosis Erdman (WT) and espK::Tn mutant cultured 3, 5, and 7 days in Sauton’s medium without detergent (A) and modified 7H9 medium without ADC and detergent supplementation (B) (each set of blots is representative of 3 independent experiments).
