FIG 4.

Secretion and expression of ESX-1 associated proteins in WT M. tuberculosis and espK::Tn cultured in liquid media with and without detergents. (A and B) Immunoblots of CF (10 μg/well) and CL (5 μg/well) total protein of wild-type M. tuberculosis Erdman (WT) and espK::Tn mutant cultured for 3 days in Sauton’s medium with and without Tween 80 (A) and Sauton’s medium with and without tyloxapol (B) to examine indicated proteins (each set of blots are representative of 3 independent experiments).