FIG 5.

Secretion and expression of ESX-1 associated proteins by WT M. tuberculosis and complemented espK::Tn. (A and B) Immunoblots of CL proteins (5 μg/well) from WT M. tuberculosis and espK::Tn strain complemented with pMD31 (empty vector), pMDespK^WT, pMDespK^W62R, and pMDespK^G64R cultured 3 days in Sauton’s medium without detergent to examine EspK levels (A) and CF (10 μg/well) and CL proteins (5 μg/well) from WT M. tuberculosis and espK::Tn strain complemented with pMD31, pMDespK^WT, pMDespK^W62R, and pMDespK^G64R cultured 3, 5, and 7 days in Sauton’s medium without detergent (B) (each set of blots is representative of 3 independent experiments).